临床神经病学杂志

目的　探讨信号转导和转录激活子 (STAT) 3在大鼠局灶性脑缺血再灌注损伤中的表达及其与缺血性神经细胞损伤的关系。方法　用 ABC免疫组化方法观察大鼠局灶性脑缺血再灌注损伤后脑组织中的STAT3蛋白免疫反应阳性细胞分布。结果　正常和假手术大鼠脑内以及脑缺血后的非缺血半球脑组织中未发现有 STAT3免疫反应阳性细胞 ,脑缺血再灌注损伤后 12小时在栓塞侧梗死区可见少量 STAT3免疫阳性细胞 ,2 4小时后阳性细胞数显著增多达高峰 ,在缺血侧纹状体和缺血皮质周边区表达最明显 ,1周后梗死周边区少数神经细胞仍有阳性表达。差异有显著意义 (P<0 .0 1)。结论　 STAT3的活化及超量表达可能介导了缺血神经细胞信号转导过程 ,并参与了脑缺血神经细胞损伤与修复的病理生理过程

Objective To explore the expression of singal transducers and activators of transcription(STAT 3) during focal cerebral ischemic reperfusive injury in rats and the relationship between ischemic neuronal damage and it.Methods Using immunohistochemical method of avidinbiotin peroxidase complex (ABC) we observed the distribution of positive cells in STAT 3 protein immunoreaction after focal cerebral ischemic reperfusive injury in rats.Results STAT 3 immunoreactive positive cells were not found in the cortex and striatum of normal and sham operative rat brains and nonischemic hemisphere brain after cerebral ischemia,small amount of STAT 3 immunity positive cells were induced in the embolism la teral infarction area 12 h after reperfusive injury,and peaked after 24 h,especially in ischemic lateral striatum and around cortex,small number of nerve cells in around infarction still showed positive expression after one week.The difference had remarkable significance( P <0 01).Conclusion The expressions of STAT 3 activation and overload might indicate the transmission course in ischemic nerve cell signal,and played a role in cerebral ischemic nerve cell injury and pathophysiological process of renovation.